Furukawa Electric Co. launched PERFLOW Sort, a new-generation flow cytometer with the novel concept in analysis and sorting of cells. It delivers high-performance & high-precision analysis using transmitted light signals as well as unique damage-less sorting at a high survival rate for living cells.
Product Catalog(PDF 157KB)Features
- Unique fixed optical fiber alignment flow cell for superior fluorescence sensitivity
- High-precision analysis using transmitted light signals
- The gentlest sorting of living cells almost without any physical damage best suitable for stem cells
- Real-time flow speed monitoring
- Optical axis adjustment free
- Available exciting light source (laser) option
- Easy-to-use benchtop instrument
- Software upgrading available
Analysis functions
- Multicolor analysis based on transmitted light and side scatter light information
- Easy-to-use management & analysis software (PERFLOW) installed as standard
- Data compatible with Flow Cytometry Standard (FCS) format
Sorting functions
- Damage-less sorting based on a mechanical method without using ultrasonic waves or high-voltage charging
- Variable cell number sorting from one to arbitrary numbers of cells per well
- Variable bulk sorting from single cell to cell cluster
- Easy aseptic setup and cleaning
- Cell deposition compatible with multi-well plates ranging from 6 to 384 wells
Specifications
| Specifications | PERFLOW Sort |
|---|---|
| Accommodated Laser | Semiconductor laser: 488nm(20mW) , 635nm(15mW) |
| Optical Parameters | 2 transmitted light(TL), 1 side scatter and 4 fluorescence |
| Fluorescence | FITC, PI (PE), PeCy5 (7-AAD), and APC |
| Sensitivity | <200 MESF FITC( when Spherotech Rainbow Beads used) |
| Data Resolution | 4 decades (Up to 5 decades) |
| Coefficient of variation | CV: below 3% (when BD QO Particles used) |
| Sensitivity of scatter detector | < 1µm to 100 µm |
| Analysis Rate | 20,000 events/second |
| Adjustment of light axis | No need |
| Sort Method | Mechanical flow changeover |
| Sort Rate | 1 event/second (for single cell sorting)* |
| Plate Deposition | Suitable for 6-384 multi-well plates and tube |
| Sort mode | Single or Enrich |
| Analysis Gates | 15 gates (Extensible) |
| Sort Purity | > 95%(single mode) |
| Standard parameter | 30 parameters (All parameters available can be saved) |
| Original parameter | Flow velocity |
| Compensation | All fluorescense |
| Available Signal | Height(log/lin), Area(log/lin) , Width(lin) , Time |
| System Pressure | 1-2 PSI |
| Other | Available to sort under sterile conditions |
| Software | sStandard control-analysis software (correspondent to FCS3.0 date format) |
| Operating System | Microsoft Windows XP Professional |
| Power | 100~240V AC |
| Size | 690×600×475mm(WDH) |
| Weight | Approx. 90kg |
Fluorescence composition option
| Number of colors | Fluorescent light |
|---|---|
| 2color | FITC, PE(PI) |
| 3color | FITC, PE, PeCy5 |
| 4color | FITC, PE, PeCy5, APC |